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苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Antigen Information
Gene Symbols:
- CCL2
- CXCL8
- IFNG
- IL10
- IL13
- IL1A
- IL1B
- IL4
- IL6
- TNF
Assay Format
Pathway:
- JAK/STAT Signaling
- MAPK Signaling
- NFkB Signaling
Number of Targets Detected:
10
Species Detected:
- Human
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Other Body Fluids
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
- Quantitative
Solid Support:
Glass Slide
Product Specifications
Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
IFN-gamma
| IL-1 alpha(IL-1 F1)
| IL-1 beta(IL-1 F2)
| IL-10
| IL-13
|
IL-4
| IL-6
| IL-8(CXCL8)
| MCP-1(CCL2)
| TNF alpha
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Inflammation Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
- Zhang C, Ding H, He M, Liu L, Liu L, Li G, et al. (2016) Comparison of Early Changes in Ocular Surface and Inflammatory Mediators between Femtosecond Lenticule Extraction and Small-Incision Lenticule Extraction. PLoS ONE 11(3): e0149503. doi:10.1371/journal.pone.0149503Species: HumanSample type: Tears
- Wu B., Wang W., Zhan Y., et al. CXCL13, CCL4, and sTNFR as circulating inflammatory cytokine markers in primary and SLE-related autoimmune hemolytic anemia. J Transl Med. 2015 Apr 8;13:112. doi: 10.1186/s12967-015-0474-4.Species: HumanSample type: Plasma
- Modulation of Cytokine Release and Gene Expression by the Immunosuppressive Domain of gp41 of HIV-1Species: HumanSample type: N/A
- Provençal, Nadine, et al. "Childhood chronic physical aggression associates with adult cytokine levels in plasma."?PLoS One?8.7 (2013): e69481.Species: HumanSample type: Plasma
- Provençal, Nadine, et al. "Differential DNA methylation regions in cytokine and transcription factor genomic loci associate with childhood physical aggression."?PLoS One?8.8 (2013): e71691.Species: HumanSample type: Plasma
- Zhai Y, Zhong Z, Chen C-YA, Xia Z, Song L, Blackburn MR, Shyu A-B. Coordinated Changes in mRNA Turnover, Translation, and RNA Processing Bodies in Bronchial Epithelial Cells following Inflammatory Stimulation. Mol Cell Biol. 2008; 28(24):7414-7426.Species: HumanSample type: N/A
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