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Recombinant Human His6-UBE2W Isoform 2 Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E2-725
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: UBE2W
Ubiquitin-conjugating Enzyme E2W (UBE2W), also known as Ubiquitin-conjugating Enzyme 16 (UBC16), is a member of the Ubiquitin-conjugating (E2) enzyme family (1). It has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (1,2). There are two isoforms of UBE2W, isoform 1 and isoform 2, with predicted molecular weights of 18.1 kDa and 19.5 kDa, respectively. UBE2W localizes to the nucleus where it promotes the mono-ubiquitination of BRCA1 and FANCD2, both of which contribute to DNA repair pathways (1-4). Mono-ubiquitination of the CHIP Ubiquitin ligase (E3), which has been reported to enhance its E3 ligase activity, is also mediated by UBE2W (5). This protein contains an N-terminal 6-His tag.
- Yin, G. et al. (2006) Front. Biosci. 11:1500.
- Alpi, A.F. et al. (2008) Mol. Cell 32:767.
- Christensen, D.E. et al. (2007) Nat. Struct. Mol. Biol. 14:941.
- Zhang, Y. et al. (2011) Mol. Cells 31:113.
- Scaglione, K.M. et al. (2011) Mol. Cell 43:599.
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsUbiquitin E3 Ligase Kits
Human CHIP Ubiquitin Ligase Kit - Glow-Fold Substrate
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