Real-time RT-PCR is made easy with the One Step PrimeScript RT-PCR Kit (Perfect Real Time), a one-step real-time RT-PCR kit designed for real-time RT-PCR (qPCR)via probe-based qPCR assays such as 5" nuclease assays¹. In this5" nuclease assay real-time PCR (qPCR) kit, one-step real-time RT-PCR can be performed in a single tube, simplifyingamplification and minimizing the risk of contamination. Amplified products are monitored in real time, so there is no need to verify them through electrophoresisfollowing PCR. The sensitivity provided by the enzymes in this kit enables detection of very small amounts of RNA, including RNA derived from viral, rare, or precioussamples.

This kit uses PrimeScript RTase,a modified, recombinant MMLV (Moloney Murine Leukemia Virus) reverse transcriptasethat is verified to have low levels of RNase H and which exhibits an excellent extension rate andquick, full-length cDNA synthesis, as well as Takara Ex Taq HS, ahigh efficiency hot start PCR enzyme optimized for this one-step real-time RT-PCR kit.