Description

Cultrex®MouseColllagen IVispurifiedfromEngelbreth-Holm-Swarm(EHS)tumor.CollagenIV isacomponentofthebasementmembranewhichisacontinuoussheetofspecializedextracellularmatrixthatformsaninterfacebetweenendothelial,epithelial,muscle,orneuronalcellsandtheiradjacentstromaandthatplaysanessentialroleintissueorganizationbyinfluencingcelladhesion,migration,proliferation,anddifferentiation.

Cultrex®MouseCollagenIV isapurifiedbasementmembraneproteinthathasbeendeveloped,producedandqualifiedforgeneralcellculturestudies.

Qualitycontrolspecifications:

• Mousecoloniesareroutinelyscreenedforpathogensusing mouseantibodyproduction(MAP)testing.
• Engelbreth-Holm-Swarm(EHS)tumorisLDEV-free.
• Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
• Endotoxinconcentration≤20EU/mlbyLimulusAmoebocyteLysate(LAL)assay.
• Proteinconcentrationof0.5mg/ml.
• NomycoplasmacontaminationdetectedbyPCR.
• Providedin50mMHydrochloricAcid,1mMTCEP–HCl.
• Purity ≥90%(SDS-PAGE).
• BIOLOGicalactivitydeterminedforeachlot:
  • TestedforABIlitytopromoteattachmentandspreADIngofratPC-12pheochromocytomacells.

Trevigen，Inc.是一家发展迅速的生物技术公司，致力于开发用于癌症研究，药物发现，遗传毒理学，再生医学和干细胞研究的产品和技术。我们提供试剂盒和试剂，用于研究DNA损伤和修复，细胞凋亡，氧化应激，癌细胞行为，3D培养和干细胞。该公司获得了美国国立卫生研究院（National Institutes of Health）多项SBIR资助。