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蚂蚁淘在线 / 品牌中心 / Trevigen / Trevigen/Cultrex®；还原生长因子基底膜基质，2型（BME 2）/3533-001-02/1 ml，2 x 5 ml，5 ml

Trevigen/Cultrex®；还原生长因子基底膜基质，2型（BME 2）/3533-001-02/1 ml，2 x 5 ml，5 ml

价格
￥700.00
货号：3533-001-02
浏览量：127
品牌：Trevigen
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商品描述

Description

Cultrex®BasementMembraneExtract(BME)isasolubleformofbasementmembranepurifiedfromEngelbreth-Holm-Swarm(EHS)tumor.Thisextractprovidesanaturalextracellularmatrixhydrogelthatpolymerizesat37°Ctoformareconstitutedbasementmembrane.Basementmembranesarecontinuoussheetsofspecializedextracellularmatrixthatformaninterfacebetweenendothelial,epithelial,muscle,orneuronalcellsandtheiradjacentstromaandthatplayanessentialroleintissueorganizationbyinfluencingcelladhesion,migration,proliferation,anddifferentiation.ThemajorcomponentsofBMEincludelaminin,collagenIV,entactin,andheparansulfateproteoglycans.

Cultrex®RGFBMEType2isaBasementMembraneMatrixthathasbeendeveloped,producedandqualifiedspecificallyforrobustorganoidcultures.Cultrex®RGFBMEType2isprocessedtoreducematrix-associatedgrowthfactorsandprovideamoredefinedmodelsystem.

Qualitycontrolspecifications:

  • Mousecoloniesareroutinelyscreenedforpathogensusing mouseantibodyproduction(MAP)testing.
  • Engelbreth-Holm-Swarm(EHS)tumorisLDEV-free.
  • PathClear®–testednegativebyPCRfor 17bacterialandvirusstrainstypicallyincludedinmouseantibodyproduction(MAP)testing,plus13additionalmurineinfectiousagentsincludingLDEV,foratotalof31organismsandviruses.
  • Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
  • Endotoxinconcentration≤8EU/mlbyLimulusAmoebocyteLysate(LAL)assay.
  • Proteinconcentrationof12–18mg/ml.
  • NomycoplasmacontaminationdetectedbyPCR.
  • ProvidedinDulbecco’sModifiedEagle’smedium(DMEM)withoutphenolred,with10µg/mlgentamicinsulfate.
  • GelstABIlitytestedforaperiodof14daysat37°C.
  • BIOLOGicalactivitydeterminedforeachlot:
    • Tubeformationassay–BMEpromotesformationofcapillary-likestructuresbyhuman(HBMVEC;HUVEC)ormouse(SVEC4-10)endothelialcells.
    • Organoidculture:BasementMembraneExtractpromotesgrowthandexpansionofmousesmallintestineorganoidProgenitorcells.
Catalog#3533-010-02includes: 
CatalogNumberDescriptionQty
3533-005-02Cultrex®ReducedGrowthFactorBasementMembraneExtract,Type2,PathClear®2
Trevigen，Inc.是一家发展迅速的生物技术公司，致力于开发用于癌症研究，药物发现，遗传毒理学，再生医学和干细胞研究的产品和技术。我们提供试剂盒和试剂，用于研究DNA损伤和修复，细胞凋亡，氧化应激，癌细胞行为，3D培养和干细胞。该公司获得了美国国立卫生研究院（National Institutes of Health）多项SBIR资助。