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CD95; APO-1; TNFRSF6; Tumor Necrosis Factor Receptor Superfamily Member 6; Apoptosis-mediating Surface Antigen FAS

Monoclonal Antibody

APO-1-3

Mouse IgG3

Purified from concentrated hybridoma tissue culture supernatant.

Recombinant human Fas.

Preservative Free

Flow CytometryImmunoprecipitationWestern BlotFunctional Application: Induces apoptosis with or without cross-linking (Protein A), depending on cell type.Note: Optimal conditions must be determined.

Human

Recognizes human Fas.

≥95% (SDS-PAGE)

1 mg/ml.

Liquid. In PBS.

BLUE ICE

+4°C

-20°C

Avoid freeze/thaw cycles.

Stable for at least 1 year after receipt when stored at -20°C.

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Fas (CD95) is a member of the death receptor (DR) family, a subfamily of the tumor necrosis factor receptor superfamily. The formation of the Fas death-inducing signaling complex (DISC) is the initial step of Fas signaling. Activation of procaspase-8 at the DISC leads to the induction of DR-mediated apoptosis. Stimulation of Fas has been also reported to trigger non-apoptotic pathways. It has been shown that membrane-bound FasL is essential for the cytotoxic activity, whereas soluble FasL appears to promote autoimmunity and tumorigenesis via induction of non-apoptotic pathways, in particular NF-κB.Product References

Monoclonal antibody-mediated tumor regression by induction of apoptosis: B.C. Trauth, et al.; Science 245, 301 (1989)

Monoclonal-antibody-mediated apoptosis in adult T-cell leukaemia: K.M. Debatin, et al.; Lancet 335, 497 (1990)

Induction of apoptosis by monoclonal antibody anti-APO-1 class switch variants is dependent on cross-linking of APO-1 cell surface antigens: J. Dhein, et al.; J. Immunol. 149, 3166 (1992)

APO-1-induced apoptosis of leukemia cells from patients with adult T-cell leukemia: K.M. Debatin, et al.; Blood 81, 2972 (1993)

The apoptosis-1/Fas protein in human systemic lupus erythematosus: E. Mysler, et al.; J. Clin. Invest. 93, 1029 (1994)

Inhibition of apoptosis in T cells expressing human T cell leukemia virus type I Tax: K.F. Copeland, et al.; AIDS Res. Hum. Retroviruses 10, 1259 (1994)

Activation induces sensitivity toward APO-1 (CD95)-mediated apoptosis in human B cells: P.T. Daniel & P.H. Krammer; J. Immunol. 152, 5624 (1994)

APO-1 (CD95) mediated apoptosis in human T-ALL engrafted in SCID mice: K.M. Lücking-Famira, et al.; Leukemia 8, 1825 (1994)

Apoptotic cell death induced by a mouse-human anti-APO-1 chimeric antibody leads to tumor regression: L.R. Coney, et al.; Int. J. Cancer 58, 562 (1994)

Cell nucleus and DNA fragmentation are not required for apoptosis: K. Schulze-Osthoff, et al.; J. Cell Biol. 127, 15 (1994)

Divergent signalling via APO-1/Fas and the TNF receptor, two homologous molecules involved in physiological cell death: K. Schulze-Osthoff, et al.; EMBO J. 13, 4587 (1994)

Autocrine T-cell suicide mediated by APO-1/(Fas/CD95): J. Dhein, et al.; Nature 373, 438 (1995)

CD40 ligation induces Apo-1/Fas expression on human B lymphocytes and facilitates apoptosis through the Apo-1/Fas pathway: E.J. Schattner, et al.; J. Exp. Med. 182, 1557 (1995)

Cytotoxicity-dependent APO-1 (Fas/CD95)-associated proteins form a death-inducing signaling complex (DISC) with the receptor: F.C. Kischkel, et al.; EMBO J. 14, 5579 (1995)

Resistance to APO-1 (CD95) induced apoptosis in T-ALL is determined by a BCL-2 independent anti-apoptotic program: K.M. Debatin & P.H. Krammer; Leukemia 9, 815 (1995)

Sensitization of T cells to CD95-mediated apoptosis by HIV-1 Tat and gp120: M.O. Westendorp, et al.; Nature 375, 497 (1995)

The medical significance of physiological cell death: G. Hacker & D.L. Vaux; Med. Res. Rev. 15, 299 (1995)

Involvement of the CD95 (APO-1/FAS) receptor/ligand system in drug-induced apoptosis in leukemia cells: C. Friesen, et al.; Nat. Med. 2, 574 (1996)

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