Trypsin,Modified,SequencingGradeTrypsin,treatedwithL-(tosylamido-2-phenyl)ethylchloromethylketonetoinhibitcontaminatingchymotrypticactivity,chemicallymodifiedtopromotestABIlityandfurtherpurifiedtoremoveautolysisfragments,resultinginahighlystabletrypsinproductresistanttoautolysiswhileretainingspecificity.Storeat-20°C.PROTECTFROMMOISTURE.REQUIRESSPECIALSHIPPING:ICEPACK≥150unitspermgprotein(atleast8,625BAEE/2875USP/NFunitspermgprotein)Source:BovinePancreas

I.U.B.:3.4.21.4

Trypsinisapancreaticserineproteasewithsubstratespecificitybaseduponpositivelychargedlysineandargininesidechains.Itisderivedfroma34kDainactiveprecursorzymogen,trypsinogen,afterenzymaticremovalofanN-terminal6-aminoacidleadersequenceresultinginthe23.8kDatrypsinmolecule.TheoptimumpHis8.0.Trypsinisinhibitedbyorganophosphoruscompoundssuchasdiisopropylfluorophosphateandnaturalinhibitorsfrompancreas.Soybean,limabean,andeggwhitearealsosourcesofnaturalinhibitors.TrypsincleavesamideandesterbondsofArgandLys.TheWorthingtonSequencingGradeTrypsinhasbeenfurtherpurifiedtoremovetracecontaminatingproteasesandautolysisproductswhichcouldinterfereintrypsindigestionexperiments,andexhibitsasinglebandonPAGE.

Uses:Fortissueculturework,Worthingtontrypsin,Codes:TRL,TRLS,TRLVMFandTRTVMFhavebeenusedbymanyresearchers.ProductCodes:TRSEQZ,TRSEQIIandTRTPCKaretypicallyusedforproteinsequencing,mappingandstructurestudies.Worthingtonmodifiedsequencinggradetrypsin,ProductCode:TRSEQZ,issubjectedtoextensivepurificationtoremovecontaminatingproteasesandtrypticautolysisby-productswhichcouldaffectthespecificityofthedigestionprocess. Subsequently,theenzymeischemicallymodifiedtominimizetheautolysisprocessaswellasincreasethestability.Themodifiedtrypsinisprocessedfurthertoremoveresidualautodegradationproducts.Thespecificityoftheenzymeisroutinelycheckedafterthechemicalmodification.

Stability/Storage:MostgradesofWorthingtontrypsinarestablefor2-3yearswhenstoredat2-8°C.Protectfrommoisture.

UnitDefinitions:TAMEUnit:OneUnithydrolyzes1µmoleofp-toluene-sulfonyl-L-argininemethylester(TAME)perminuteat25°C,pH8.2,inthepresenceof10mMcalcium. 

Note:1mgtrypsin≥180TAMEunits,10,350BAEEunits,3,450USP/NFunits.

TechnicalNotes:TheVirusandMycoplasmaFreetrypsin(Code:TRTVMF)hasbeenfilteredthroughan0.22micronporesizemembrane,lyophilized,subjectedtogammairrADIation,andtestedforvirusandmycoplasma.

RelatedProducts:

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• Hyaluronidase(HSE/HSEP)
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美国Worthington Biochemical Corporation成立于1947年，位于美国东海岸美丽海滨小城Lakewood, 有超过60年的生产经营历史，是世界上最主要的酶类供应商，是细胞分离酶的专家级公司。同时，也提供DNA/RNA纯化等分子生物学方面的产品。Worthington已经被购买和出售了好几次，并于1987年返回到沃辛顿家族。60年来，Worthington一直保持世界上高纯度酶的主要来源。