Overview

Nocodazoleisananti-mitoticagentthatreversIBLyinterfereswiththepolymerizationofmicrotubules(DeBrabanderetal.).Nocodazolebindstobeta-tubulinanddisruptsmicrotubuleassembly/disassemblydynamics,impairingformationofthemetaphasespindlesduringthecelldivisioncycle.ThispreventsmitosisbyinducingaG2/M-phasearrestandinducesapoptosisintumorcells(Jordanetal.).GENOMEEDITING·Synchronizeshumanpluripotentstemcells(hPSCs)intheG2/Mcellcyclephase,therebyincreasingtheefficiencyofhomology-directedrepair(HDR)inCRISPR-Cas9genomeediting(Linetal.;Yangetal.).CANCERRESEARCH·InducesapoptosisinleukemiccellswithoutharmingTcellsand/ormesenchymalstromalcellsthatwererecoveredfromthesamepatients(Frezzatoetal.).